Justice Department officials intend to interview senior C.I.A. officers as they review the Russia investigation, according to people briefed on the matter, indicating they are focused partly on the intelligence agencies’ most explosive conclusion about the 2016 election: that President Vladimir V. Putin of Russia intervened to benefit Donald J. Trump.

The interview plans are the latest sign the Justice Department will take a critical look at the C.I.A.’s work on Russia’s election interference. Investigators want to talk with at least one senior counterintelligence official and a senior C.I.A. analyst, the people said. Both officials were involved in the agency’s work on understanding the Russian campaign to sabotage the election in 2016.

While the Justice Department review is not a criminal inquiry, it has provoked anxiety in the ranks of the C.I.A., according to former officials. Senior agency officials have questioned why the C.I.A.’s analytical work should be subjected to a federal prosecutor’s scrutiny. Attorney General William P. Barr, who is overseeing the review, assigned the United States attorney in Connecticut, John H. Durham, to conduct it.